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This topic was originally posted in this forum: Miscellaneous Forum |
| Author | Topic: Ricin |
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Feticidal Fantasy Frequent Poster |
Does anyone know how to extract/concentrate ricin from castor bean seeds? Castor bean seeds contain 5% ricin, and I would like to know how to get rid of the extra 95%. Thank you very much if you can help me out. ------------------ |
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Ho ju Moderator |
what praytell is ricin? is it a poison? ------------------ |
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Apathetic Frequent Poster |
This comes from the "Poor Mans James Bond 3" If you can get ahold of kurt saxon's video PMJB greets the russians he shows you on the video how to do it. " To get the best powder you want to remove the hulls of the bean. Do this by putting 2 ounces of water in a glass and pour in a teaspoonful of lye. wait until it cools then put in one or two ounces of beans. they float so put a weight on top of them to hold them under.soak for one hour. then wash them thoroughly and dry them in a towel. the hulls expand and can be cracked along the sides with a fingenail (on the video he uses needle nose pliers).put the hulled beans in a glass or metal blender with four ounces of acetone to each ounce of beans (4 to 1).blend them until they are the consistency of milk and put it in a glass jar with a lid for three days then swirl well and pour the "brew" into a coffee filter that is in a glass,opaque plastic or metal funnel. when the dripping stops, take out the filter and gently squeeze out the remainder of acetone. spread the filter on a newspaper and let it dry. although most of the oil is out of the pulverized beans some of it may be picked up again as the acetone- oil mixture goes through the pulp while its in the filter. if after the acetone has evaporated from the powder, it still holds together after being compressed, it still has too much oil in it. put it back in the jar and add four more ounces of acetone. swirl well and let it set another day.repeat the process and you should have pure, oilless powder. don't let this get up your nose or the noses of any one you dont want out of the way." |
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Feticidal Fantasy Frequent Poster |
is the kurt saxon cook book good information or it is false? I read a method very similar to that on an anarchy site, they probably copied it from that. Ho Ju, ricin is a very very potent toxin. it only takes a single bean to kill a baby, 5 - 8 beans for an adult. it is absored through the intestine walls. the toxin is found in the endosperm of the seed. it is almost totaly untraceable in optopsy, even when it is suspected. it is pretty cool shit. ------------------ |
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Survivor Frequent Poster |
This article comes from Uncle Fester's excellent book "Silent Death": RICIN: KITCHEN IMPROVISED DEVASTATION For those unable or unwilling to tackle the more technically demanding tasks of nerve gas manufacture or botulin culture, Mother Nature's bounty has provided a considerably more low-tech alternative: Ricin. Ricin is an exceedingly toxic protein found in the castor oil bean. One pound of pure ricin is a lethal dose for over three million people if delivered by injection. If inhaled in the form of a fine dust, it is nearly as potent. Eating ricin also produces a lethal result, although the dose must be considerably higher due to partial destruction of the poison in the digestive tract. In mass-attack situations, ricin has one great advantage over nerve gas. This advantage is a delay in the onset of symptoms of poisoning of 12 to 24 hours. As a result, a target most likely will not realize it is under attack Further, once symptoms do begin to appear in the unfortunate targets, it will be very difficult for investigators to back-track to the site of the original attack. These two factors combine to greatly increase the likelihood of success for the attack mission. For assassination, ricin is similarly well-endowed with favorable qualities. There is no antidote or treatment for ricin poisoning. A lethal dose produces death, period. The long delay between administration of the poison and symptoms means that the mark will very likely not be able to make the connection to where or from whom he met his demise. Treatment and autopsy investigations are further frustrated by the fact that ricin leaves no traces of itself in the body, and there is no test for it. A couple of hours after administration of the poison, blood from a poisoned animal can be transfused into another animal with no harm to the receiving animal. This is because all the ricin has already passed from the bloodstream into individual cells. Pretty much the only way the ricin poisoning could be identified positively as such is if mushedup whole castor beans are fed to the mark. Then close examination of the resulting Hershey squirts will probably find fragments of the beans. A refined extract will not be detectable. A tale from the heydays of the Cold War is instructive on this matter. Ricin was a favored assassination too[ of the KGB. One person who became a target of their wrath was a defector from the Eastern Bloc who fled to England where he proceeded to make trouble for his former oppressors. To silence him, a KGB operative walked up behind him on the crowded street, and poked him in the back of his leg with the tip of his umbrella. This specially rigged umbrella then injected into the ill-fated defector a small metal ball with a hollow core packed with ricin, and a couple of holes in the ball to allow for blood circulation to dissolve the ricin and release it into the blood stream. Within a day, the doomed man made his way to a hospital, where his condition continued to deteriorate. Given his position, he rightly suspected that he was the victim of foul play, not the flu or some bad sausage. Run whatever tests they might, however, the doctors couldn't get a clue as to what his problem was Finally, X-rays revealed the small metal ball in his leg, and it was removed. Traces of ricin were found still on the metal, but nothing could be done to save him. Had this metal ball not been found, the cause would never have been determined. The spectacular toxicity of ricin is due to its catalytic, rather than stoichiometric, mechanism of poisoning. The ricin protein molecule is constructed of two separate protein chains, called A and B chains. The function of the B chain is to bind to the surface of a cell, and inject into the cell the A chain. The A chain then goes from ribosome to ribosome within the cell, inactivating each, making them incapable of producing proteins for the cell to use. The A chain isn't consumed or otherwise effected in this process, so it's possible for one molecule of ricin to kill an entire cell. The symptoms of ricin poisoning will vary with the method of ingestion. When it has been eaten, vomiting and the runs are very prominent, along with weakness and fever. Eventually, convulsions set in, and the person dies when the area of the brain that controls breathing stops working. When the substance has been breathed in or injected, then the puking and squirts aren't as pronounced. The symptoms of poisoning by ricin are quite variable from person to person, and this makes diagnosis of the problem very difficult. To produce ricin, one first needs a supply of the castor beans which contain the ricin. How to best get these castor beans depends upon how much ricin is needed for the desired application. The typical concentration of ricin within the beans is in the area of 7/10 of 1%, through extraction and a series of purification steps the yield is about half of that. Smaller amounts of beans can be purchased through seed dealers, or Kurt Saxon's company: Atlan Formularies. Larger amounts should only be obtained by growing a crop. The castor bean plant will grow to maturity throughout the Southern US. It prefers fertile soil with reasonable amounts of moisture. Corn or cotton fields can easily be converted to castor bean production. A short listing of seed dealers who carry castor beans follows: Atlan Formularies Mellinger's Inc. Joe Harris Hudson Seedsman Stokes Seeds, Inc. There are a numbers of varieties of the castor bean plant (Ricinus communis) from which one may choose. Generally, most of the varieties have only small cosmetic differences from one another that are unimportant to the end use. However, dwarf varieties such as Ricinus communis gibsonii should be avoided, as the resulting small beans require too much labor to hull prior to extraction. The typical castor bean is about the size of one's thumbnail. In addition to being able to purchase castor beans from established seed sources, it may not be too difficult or suspicious to order a reasonably large quantity through a "feed and seed" retailer. It's probably wiser not to express interest directly in the castor oil crop, which is rare in most areas, but to request castor beans as a cover crop to benefit a cash crop which is common in your area. This will only ring true if you live in a warm region of the country, however. Further, if you happen to live in or travel to Florida, South Texas or Southern California, some retailers will probably have castor beans in stock during the planting season because it is in these regions that the small castor oil industry still exists. Hybrid varieties of castor bean are superior in respect to crop yield, disease resistance, and robust growth. They are, however, a poor choice for this project because one would want to use some or all of the beans to have a larger crop in the following year. Since the seeds (beans) derived from hybrids tend to have a low germination rate and unpredictable qualities, one is better advised to stick to truebred or inbred varieties instead. By planting the many beans obtained from planting a single bean and letting the plant mature, one can, over a period of time, parlay even a small initial acquisition of castor beans into a large crop. In tropical regions, the castor bean plant is a robust perennial that can reach 15 feet in height, and take on the proportions of a large shrub. In areas subject to frost, it can grow as an annual. It should be planted just after the danger of frost has passed, about 23 inches deep in fertile well-drained soil with full sunlight. When the plant matures, it bears fruits which are capsules covered with long, soft spines. These fruits contain the castor beans. To extract the ricin from the beans, one should first remove the hulls from the seeds. This is a very labor-intensive task, and is hard on the fingers. The hulls can be made easier to remove by first soaking the beans in a solution made up of one 12 oz. can of lye dissolved in a 1/2 gallon of water. The soak should last for about one hour, then wash off the beans and spread them out to dry. This will help, but it's still not easy. If any of you know of a good tool for the task, feel free to write with suggestions. Once the hulls of the beans are removed, one is left with the soft white meat of the beans. The castor oil must next be removed from the product. Acetone is probably the best solvent to use for this purpose, as it is easily available at the hardware store and the residue quickly evaporates from the bean pulp. Hexane is also a good choice if it is available to you. To remove the oil from the pulp, first mush up the beans with a hammer and load the bean meats into a blender until it is half filled. A plastic blender pitcher shouldn't be used with acetone as it will be dissolved. A metal blender pitcher should be used with this solvent. Now add acetone or hexane to the mushed-up beans until the blender pitcher is just about full. Then put on the cover and blend until a smooth, milky mixture is obtained. Pour this mixture into a covered glass container, and let it sit for a few hours, then filter through a coffee filter. Squeeze out as much solvent as possible from the bean pulp, either by packing with a spoon, or even better by using a Buchner funnel and filtering flask attached to a line of vacuum. Next return the bean pulp to the blender, and add a fresh load of solvent. Blenderize the pulp as before, and filter the mixture. Once the pulp has been drained of solvent, repeat the solvent extraction. Then it should be spread out to dry in an area without much wind movement, as breathing in the dust would be bad. When the pulp has dried of solvent, it should have a chalky, talcum-like consistency. If it still seems oily, it should be extracted again with solvent. If extraction of the ricin from the defatted bean pulp isn't going to be done right away, the powder should be stored in a freezer. Always wear a very good dust mask when handling this powder. Everything which comes in contact with the bean powder should be washed very thoroughly or destroyed. To extract the ricin from the pulp, I direct the reader to several references. All of them are relevant. See US patent 3,060,165. Also see European Journal of Biochemistry, Volume 105, pages 453-5 (1980) and Biochemistry, Volume 12, No. 16, pages 3121-26 (1973). An article from Toxicology, Volume 2, pages 77-102 (1974) is also interesting. For many examples of how not to go about this procedure, see The Weaponeer and The Survivor by Kurt Saxon. His bumbling and fumbling of the extraction and isolation is almost comical, and very instructive. He ultimately gave up, since he was only able to produce small amounts of a weak product. I really shouldn't make fun of him, since his formal chemistry training is obviously rudimentary. Rather, the model which will be followed here is that of Dr. Albert Hoffmann, the discoverer of LSD, and the experimental tactics he used to isolate and find the molecular structure of psilocybin, the active ingredient of fumy mushrooms. Dr. Hoffmann was able to far outpace his competitors in the race to isolate and identify psilocybin because he an the members of his research team were willing to taste-test their extracts to assure that the active ingredient was being isolated. This idea never occurred to his competition, as they were too chicken shit and too tied-up with the establishment to contemplate such a course. Obviously, taste-testing one's extracts will bring this project to a rapid and fatal conclusion. For this job, one should recruit a series of white lab rats, available at the pet store as food for your boa constrictor. This project will keep him quite hungry. The extraction of ricin from castor bean pulp is a pretty standard protein extraction, very similar to procedures used to extract and isolate other proteins from a plant matrix. The first and most important point to keep in mind is that the extraction must be done at refrigerator temperatures. This prevents other enzymes extracted out of the bean from degrading the product ricin in solution. Exact control of the pH during extraction doesn't appear to be so crucial to success, as the European Journal of Biochemistry article used full-strength vinegar (5% acetic acid) as the extractant. This gives an extraction pH of under 3. Both the US patent and the Biochemistry article preferred a pH of 3.8 to 4, with the Biochemistry article using dilute vinegar to reach that pH, and the patent using dilute sulfuric acid. I am mildly suspicious of the patent, as I feel it may contain some deliberate misinformation. Beyond that, it was filed in 1952, and kept secret until 1962, so their procedure represents antique technology. The procedure followed here is a melding of the three procedures. The defatted, solvent free bean pulp obtained from one kilo of castor bean meats (it now weighs considerably less than a kilo with the oil removed) is suspended in 4000 ml of distilled water cooled to about 4C (refrigerator temperature). Then, with stirring, add white distilled vinegar until the pH reaches 3.8 to 4. A good, accurate, properly calibrated pH meter is best for measuring pH. The beans must be thoroughly defatted to get good extraction, so don't skimp on the solvent in the previous step. The oil would act to prevent good wetting of the bean pulp and prevent extraction. Now let this mixture sit in the fridge overnight. When morning comes, pour it into a blender, and while it's still cold, grind it hard, at maximum speed, for a minute or two, then return it to the fridge, where it should be magnetically stirred for 24 hours. Place a layer of cardboard between the stirrer and the product to prevent heat transfer from the stirrer. Now it is time to separate the vinegar extract from the bean sludge. Filtration won't work very well, because it's just too gooey. Centrifugation is far superior. The articles recommend a centrifuge speed of 10,000g for 30 minutes. The extract must be kept cold. Work outside in the winter, or in a walk-in freezer in order to maintain proper temperature. The clear-colored vinegar extract should be separated from the centrifuge-compressed bean sludge (termed a pellet), and stored in the refrigerator. The pellet should then be extracted with one more portion of dilute vinegar at pH 3.8 to 4 at a temperature of 0-4° C with stirring overnight. This extract should then be similarly obtained by centrifugation. The US patent claims that the extract can be separated from the bean sludge by filtration, if a filter aid such as Celite 503 is added to the bean sludge-vinegar mixture to the extent that it is about 10% by weight of the defatted bean meal used. Your comments on this variation are welcome. Now the combined dilute vinegar extracts, while still kept cold, are gradually brought to 100% saturation with solid ammonium sulfate. This requires about 70 grams of ammonium sulfate per 100 nil of extract. It should be added slowly, with stirring. This precipitates the ricin out of solution. Once saturation has been reached, keep the solution overnight in the refrigerator. The precipitated crude ricin is now collected by centrifugation. The article recommends 23,000g for two hours. The product is the pellet. Remove the supernatant vinegar, collect the pellet, and dry it under a vacuum. Now our animal friends come into play to test the extraction process. Feed some of them bean sludge residue to test for completeness of extraction. Feed some of them supernatant vinegar to test for completeness of precipitation, and feed some of them your crude product. Varying degrees of dilution are called for to judge just how much of this very potent poison has been wasted, and how much has been captured. This vacuum-dried, crude ricin is likely to be good enough for most practical purposes; however, all of the articles move onward to get pure ricin. The scientific articles use chromatography, while the patent uses another extraction and precipitation step. Let's follow the patent here. Suspend the still-wet crude ricin pellet in three times its weight of distilled water, or suspend the vacuumdried ricin in 30 times its weight of distilled water. The water should be cold, and kept cold. Now dropwise, with strong stirring, add 5% by weight sulfuric acid solution until a pH of 3.8 is reached. Stir for about an hour, then filter. Then to the filtrate, dropwise add 12% sodium carbonate solution until the pH rises to about 7. Now the pure ricin can be precipitated by slowly adding a solution made up from two pounds of sodium sulfate dissolved in 10 pounds of water to the neutral ricin solution until the amount of sodium sulfate added equals 20% of the original weight of the neutral ricin solution. For instance, let's say you have about a pint of this ricin solution. It weighs about a pound. You will want to add sodium sulfate solution to it until you have added about 90 grams of sodium sulfate. This requires adding about 540 ml of sodium sulfate solution. Stir in the cold for an hour or so to complete precipitation, then filter. The filter cake should be rinsed with some more of the sodium sulfate solution to remove more non-toxic proteins. Just one good rinse is called for here, as more does no good. Finally, vacuum dry the filter cake. This produces a product which is about half pure ricin, and half solid sodium sulfate. The sodium sulfate does no harm to the ricin. However, for use as an airborne weapon of devastation, it's necessary that the size of the ricin particles be reduced to a very small size, on the order of a few microns, which is similar to smoke particles. The small size is needed so that the particles remain airborne, rather than settling out as dust. Also, the small size is needed to make sure that the particles get all the way down into the lungs, rather than being caught in the snot-filled slime that covers one's air passages. The patent addresses this problem, and says that the best results were obtained with air grinding. Putting the dried ricin into a blender and letting the blades whip it up and grind it would seem to me to be to be analogous. To prevent detoxification of the ricin, it must be quite free of water when it is ground, and kept cold. They also suggest ball-and-hammer milling of dried ricin in a slurry of CCl4. Keening it cold is also important here. Spray-drying a 20% solids slurry with CC14 was also used. See the patent. Once ground, all one needs is a fan to propel airborne death on its way to the target. A really good method for getting a small ricin particle size can be used if the plan is to spray the enemy territory from some height, as from the top of a reasonably tall building or from a low-flying plane. This method is to dissolve the ricin into water adjusted to pH 4 with acetic acid or vinegar. No more than one gram of ricin should be dissolved in each liter of water. Then when this mixture is sprayed from a reasonable height, the water in each droplet will evaporate as it falls through the air, leaving a tiny particle of ricin to float on the wind. Naturally, the use of this plan requires that weather conditions be favorable for evaporation of the water droplet. This simple procedure works well. One caveat, however: this solution will begin to grow a bustling bacteria population in no time flat. Within a day or so, floating strands of slimey gunk will be in solution, ready to plug up the sprayer. Worse still, the bacteria may well be able to digest the ricin, rendering it harmless. For this reason, the solution must be used quickly. Happy hunting! WARNING.. Possession of refined ricin will likely get you a life term! Beyond experimental runs to perfect your technique, these procedures should only be done under war situations. If stockpiling must be done, it should only be in a very secure place, with freezing temperatures to assure freshness of the product. ------------------ |
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nbk2000 Frequent Poster |
The one time I tried this what I did was extract the oil using acetone, and after filtering, precipitating the ricin from the solution using epsom salt (magnesium sulfate). It worked just fine but you don't get very much, probably less than 5% of the total weight of beans. But considering how toxic it is you don't need very much. The one thing that is very important to remember is that any heat will deactivate the toxin. Cold soda, ice cream, cold sandwhichs, etc. are the foods to use. I'm not sure how practical the fester method is since it relies on a centrifuge. How common are those? ------------------ |
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Bandit Frequent Poster |
The fist bit of this file was from anarky today #2 but i have not included it here. POISON [1] makes most of these points: * Most drugs cause vomiting. To help stop this, take one or two anti- Common drugs: * Cyanide (HCN, KCN) [3]: "Hydrocyanic acid is one of the most poisonous substances [1, DGHS talking about KCN]: * Aspirin (acetylsalicylic acid) * Paracetamol (aka acetaminopren / tylenol) * Sleeping tablets (see specific notes for each kind) * Alcohol (spirits preferably, your choice) * Water Calle: The above anecdote originally came from me, and the death * Bleach and other corrosives (lye, drain cleaning fluids) * Insulin (injected) * Petrol (in lungs/injected) * Oil of Wintergreen/Methyl Salicylate (in lungs/injected) * Malathion (insecticide) (entry revised by Calle) * Phosphine gas from aluminium phosphide pesticide (ALP) * Rat poison (Warfarin) Calle: Since human haemophiliacs usually live quite ordinary * Caffeine * Potassium Chloride (injected in solution) / KCl * Nitrogen gas (or other inert gas) * Nitrous oxide (N20? NO2?) * Carbon Monoxide (CO) Calle: A correspondent from Denmark, where you still can use The actual cause of death is asphyxiation, since the carbon * Chlorine gas * Hydrazine * Chloroform * Digitalis (Foxglove, Digitalis Purpurea) * Yew (Taxus Baccata, the "English Yew")
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Bandit Frequent Poster |
I have a large file on all sorts of poisons and their effects. The forum wont let me post it, so email at xtreme52@hotmail.com Bandit |
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Ho ju Moderator |
is ricin pronounced with a hard c as in rikin or soft as in riCEin ------------------ |
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megalomania Administrator |
Its the soft, like rye sin ------------------ |
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Ho ju Moderator |
thanks ------------------ |
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